Description

Succinimidyl-2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylate is a highly reactive, spin-labeled N-hydroxysuccinimide (NHS) ester derivative, serving as a critical bioconjugation reagent for site-specific protein and peptide labeling. Its core value lies in introducing a stable nitroxide radical (TEMPO) tag, enabling advanced structural and dynamic studies via Electron Paramagnetic Resonance (EPR) spectroscopy without significantly altering the biomolecule's native function. This compound is essential for researchers and manufacturers in biopharmaceutical R&D, structural biology, and diagnostic assay development who require precise spin labeling for probing protein conformation, interactions, and dynamics.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Primary application for studying protein structure, folding, and conformational changes.
• Bioconjugation & Protein Labeling: Covalently attaches a stable nitroxide radical tag to primary amines (e.g., lysine residues or N-termini) on proteins, antibodies, and peptides.
• Membrane Protein Studies: Used to label and investigate the dynamics and topology of integral membrane proteins.
• Molecular Ruler Applications: Measures intramolecular distances in biomolecules via Double Electron-Electron Resonance (DEER) or Pulsed EPR techniques.
• Diagnostic Probe Development: Creation of spin-labeled probes for immunoassays and biosensors.
• Polymer & Material Science Functionalization: Introduction of radical labels onto amine-functionalized surfaces or polymers for advanced material characterization.
• Drug Discovery & Biophysics: Tool for screening protein-ligand interactions and understanding molecular mechanisms.

Basic Information

Product Name	Succinimidyl-2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylate
CAS No.	58537-73-8
Molecular Formula	C14H19N2O5
Molecular Weight	295.31 g/mol
Synonyms	3-Carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl N-hydroxysuccinimide ester; TEMPO NHS Ester; 3-Carboxy-PROXYL NHS ester; Succinimidyl 2,2,5,5-tetramethyl-1-oxyl-3-pyrroline-3-carboxylate; 3-Carboxy-2,2,5,5-tetramethylpyrrolidin-1-oxyl N-succinimidyl ester; SL-NHS; Spin-label NHS ester; 1-Oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-carboxylic acid N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our Succinimidyl-2,2,5,5-Tetramethyl-3-Pyrroline-1-Oxyl-3-Carboxylate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and radical content. We adhere to cGMP principles for production and control, ensuring reliability for critical bioconjugation workflows. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed container with silica gel) due to its hygroscopic and moisture-sensitive nature. For long-term storage, keep under an inert atmosphere (argon or nitrogen) to preserve radical stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Radical Content (EPR)	≥ 90% of theoretical
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.