Description

5-Methylcytosine Hydrochloride is a modified nucleoside derivative of cytosine, where a methyl group is attached at the 5-position of the pyrimidine ring. This compound is a critical biochemical reagent and building block in the field of epigenetics and nucleic acid research. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and biotechnology sectors for studying DNA methylation, developing diagnostic tools, and synthesizing modified oligonucleotides.

Application

• Epigenetics Research: A fundamental substrate for studying DNA methylation processes, gene expression regulation, and epigenetic modifications.
• Pharmaceutical Development: Serves as a key intermediate in the synthesis of novel nucleoside analogs and potential therapeutic agents targeting epigenetic pathways.
• Diagnostic Reagent Production: Used in the manufacture of kits and reagents for detecting DNA methylation patterns, which are biomarkers for various diseases including cancer.
• Biochemical Synthesis: An essential building block for the chemical and enzymatic synthesis of methylated oligonucleotides and DNA probes.
• Academic & Industrial R&D: Employed in laboratories for fundamental biochemical studies, assay development, and high-throughput screening.
• Reference Standard: Used as a certified reference material (CRM) for analytical method development and validation in quality control laboratories.

Basic Information

Product Name	5-Methylcytosine Hydrochloride
CAS No.	58366-64-6
Molecular Formula	C5H8N3O·HCl
Molecular Weight	177.60 g/mol
Synonyms	5-Methylcytosine HCl; 4-Amino-5-methyl-2(1H)-pyrimidinone hydrochloride; 5-MC·HCl; 5-Methylcytosinium chloride; Cytosine, 5-methyl-, hydrochloride; 5-Methyl-2-oxo-4-amino-1,2-dihydropyrimidine hydrochloride
EINECS	Contact for details

Quality Control

Our 5-Methylcytosine Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture content. We provide full traceability and Certificates of Analysis (COA) are available for all shipments, ensuring compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥98.0%
Water Content (KF)	≤2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.