Description

5-Bromo-3-Indolyl-2-Acetamido-2-Deoxy-β-D-Glucopyranose is a specialized glycoside derivative that serves as a critical chromogenic substrate for enzymatic detection. Its value lies in its high specificity and sensitivity for detecting β-glucosidase and related hydrolase activity in biochemical assays. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors who require reliable reagents for histochemistry, microbiology, and molecular biology applications.

Application

• Enzyme Substrate for Diagnostics: Used as a chromogenic substrate for the detection and quantification of β-glucosidase activity in diagnostic kits and clinical assays.
• Microbiological Research: Employed in microbial identification and characterization studies to detect specific enzymatic pathways.
• Histochemistry & Cell Biology: Applied in staining procedures to localize enzyme activity in tissue sections and cell cultures.
• Biochemical Reagent: Serves as a key component in research-grade reagent formulations for studying glycosidase mechanisms and inhibition.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for drug discovery.
• Food & Environmental Testing: Can be applied in assays designed to detect microbial contamination or specific enzymatic activities in samples.

Basic Information

Product Name	5-Bromo-3-Indolyl-2-Acetamido-2-Deoxy-β-D-Glucopyranose
CAS No.	58225-98-2
Molecular Formula	C₁₆H₁₉BrN₂O₇
Molecular Weight	431.24 g/mol
Synonyms	5-Bromo-3-indolyl N-acetyl-β-D-glucosaminide; 5-Bromo-3-indolyl 2-acetamido-2-deoxy-β-D-glucopyranoside; X-GlcNAc (5-Bromo); 5-Br-3-indoxyl N-acetyl-β-D-glucosaminide; 5-Bromo-3-indoxyl β-D-N-acetylglucosaminide; NAG-5-Br-3I; 5-Bromo-3-indolyl β-D-GlcNAc; Chromogenic β-N-acetylglucosaminidase substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indolyl-2-Acetamido-2-Deoxy-β-D-Glucopyranose is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant guidelines for fine chemical production to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Performance	Yields characteristic blue indigo dye upon enzymatic cleavage

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.