Description

p-Nitrophenylphosphate, Ammonium salt is a high-purity biochemical reagent widely used as a substrate for alkaline phosphatase in diagnostic and research applications. Its primary value lies in its ability to produce a quantifiable yellow color upon enzymatic hydrolysis, enabling sensitive colorimetric detection and assay development. This compound is essential for professionals in the in-vitro diagnostics (IVD), pharmaceutical research, and life science research sectors who require reliable and consistent performance in enzymatic assays.

Application

• Primary Substrate for Alkaline Phosphatase (ALP): Used in ELISA, immunoblotting, and other immunoassay formats for signal generation.
• Clinical Diagnostics: A key component in reagent kits for the automated colorimetric determination of ALP activity in serum, a critical marker for liver and bone disorders.
• Molecular Biology Research: Employed in non-radioactive detection systems for nucleic acid hybridization (e.g., Southern, Northern blotting) using enzyme-conjugated probes.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) assays to identify modulators of phosphatase activity for drug discovery.
• Quality Control in Biotechnology: Serves as a standard reagent for testing the activity and consistency of alkaline phosphatase enzyme preparations.
• Food Safety Testing: Applied in certain enzymatic methods for the detection of specific contaminants or biomarkers.

Basic Information

Product Name	p-Nitrophenylphosphate, Ammonium salt
CAS No.	57885-61-7
Molecular Formula	C6H9N2O6P
Molecular Weight	236.12 g/mol
Synonyms	4-Nitrophenyl phosphate ammonium salt; PNPP, Ammonium salt; p-Nitrophenyl phosphate diammonium salt; Phosphoric acid 4-nitrophenyl ester diammonium salt; 4-NPP; ALP Substrate; Alkaline Phosphatase Substrate; Di-ammonium 4-nitrophenyl phosphate; Nitrophenylphosphoric acid ammonium salt
EINECS	Contact for details

Quality Control

Our p-Nitrophenylphosphate, Ammonium salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent enzymatic activity, and low background absorbance, which are critical for sensitive assay performance. Certificates of Analysis (COA) providing detailed specifications for HPLC purity, assay, absorbance characteristics, and water content are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance and stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	Suitable for use as ALP substrate
Absorbance (1% Solution)	Meets typical limits for low background
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.