Description

4-Methylumbelliferylguanidinobenzoate is a specialized fluorogenic substrate and biochemical reagent of significant importance in enzymatic research and diagnostics. Its core value lies in its ability to serve as a sensitive and selective probe for detecting specific protease activity, particularly for enzymes like trypsin and urokinase-type plasminogen activator. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic kit development sectors, where precise enzyme quantification is critical for drug discovery, biochemical assays, and clinical testing.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and kinetic study of serine proteases, including trypsin and thrombin.
• Diagnostic Reagent Manufacturing: Key component in the formulation of diagnostic kits for measuring proteolytic enzyme levels in clinical samples.
• Drug Discovery & High-Throughput Screening (HTS): Used in pharmaceutical research to identify and characterize novel protease inhibitors as potential therapeutic agents.
• Biochemical Research: Employed in academic and industrial laboratories to study enzyme mechanisms, inhibition constants (Ki), and catalytic efficiency.
• Quality Control in Bioprocessing: Monitors protease activity during the production and purification of biopharmaceuticals to ensure product consistency and purity.

Basic Information

Product Name	4-Methylumbelliferylguanidinobenzoate
CAS No.	57817-70-6
Molecular Formula	C19H17N3O5
Molecular Weight	367.36 g/mol
Synonyms	4-Methylumbelliferyl 4-guanidinobenzoate; MUG-benzoylguanidine; 4-Guanidinobenzoic acid 4-methylumbelliferyl ester; Fluorogenic trypsin substrate; 7-Hydroxy-4-methylcoumarin-4-guanidinobenzoate; 4-MUGB; Boc-Gly-Gly-Arg-AMC (related class); Trypsin substrate MUGB
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferylguanidinobenzoate is produced under strict quality management systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorogenic Activity	Functional in standard trypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.