Description

3,3`-Dithiobispropanoic Acid Bis(n-Hydroxysuccinimde Ester) is a high-purity, heterobifunctional crosslinking reagent featuring a cleavable disulfide bridge and two amine-reactive NHS ester groups. This compound is critical for creating reversible, reducible linkages in bioconjugation strategies, enabling the development of stimuli-responsive biomaterials and drug delivery systems. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceuticals, proteomics, and advanced material science for applications requiring controlled release or site-specific cleavage.

Application

• Bioconjugation and Crosslinking: For reversible covalent attachment of peptides, proteins, antibodies, or other amine-containing biomolecules.
• Controlled Drug Delivery Systems: As a key linker in antibody-drug conjugates (ADCs) or nanoparticle formulations designed for glutathione-mediated release in target cells.
• Proteomics Research: In the synthesis of cleavable crosslinkers for mass spectrometry analysis of protein structures and interactions.
• Surface Functionalization: To create reducible coatings on biosensors, chips, or medical devices for controlled biomolecule immobilization.
• Polymer Chemistry: As a building block for synthesizing smart, stimuli-responsive hydrogels and polymers.
• Diagnostic Assay Development: For constructing reversible capture surfaces in immunoassays or other diagnostic platforms.

Basic Information

Product Name	3,3`-Dithiobispropanoic Acid Bis(n-Hydroxysuccinimde Ester)
CAS No.	57757-57-0
Molecular Formula	C14H16N2O8S2
Molecular Weight	404.42 g/mol
Synonyms	DTSSP; 3,3'-Dithiobis(sulfosuccinimidyl propionate) (sulfonated analog); Bis(sulfosuccinimidyl) 3,3'-dithiodipropionate; NHS-DTSSP; Dithiobis(succinimidyl propionate); DSP (Dithiobis(succinimidyl propionate) analog); 3,3'-Dithiodipropionic acid bis(N-hydroxysuccinimide ester); Bis(N-succinimidyl) 3,3'-dithiodipropionate
EINECS	Contact for details

Quality Control

Our 3,3`-Dithiobispropanoic Acid Bis(n-Hydroxysuccinimde Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.