Description

(20R)-5β-Pregnane-3α,11β,17,20,21-Pentol Tris(Trimethylsilyl) Ether CAS NO 57325-69-6 is a high-purity, derivatized steroid reference standard, specifically a pentol silyl ether. This compound is critically important for analytical and research applications where precise quantification and identification of steroid metabolites are required. It is primarily needed by pharmaceutical R&D laboratories, clinical research organizations, and manufacturers of diagnostic kits for use as an internal standard or calibration standard in sophisticated chromatographic and mass spectrometric analyses.

Application

• Analytical Reference Standard: Primary use as a certified reference material (CRM) for the quantification of steroid hormones and their metabolites via GC-MS or LC-MS/MS.
• Pharmaceutical Research: Used in the development and validation of bioanalytical methods for pharmacokinetic and metabolism studies of steroid-based drugs.
• Clinical Diagnostics: Serves as a critical component in the calibration of assays for endocrine disorder testing, such as congenital adrenal hyperplasia (CAH) or cortisol pathway analysis.
• Metabolomics: Employed in research studies investigating steroidogenesis and metabolic pathways in both clinical and pre-clinical settings.
• Quality Control: Used by in-vitro diagnostic (IVD) manufacturers to ensure the accuracy and reliability of their steroid hormone test kits and automated immunoassay systems.

Basic Information

Product Name	(20R)-5β-Pregnane-3α,11β,17,20,21-Pentol Tris(Trimethylsilyl) Ether
CAS No.	57325-69-6
Molecular Formula	C32H64O5Si3
Molecular Weight	613.1 g/mol
Synonyms	5β-Pregnane-3α,11β,17,20,21-pentol, tris(trimethylsilyl) ether; 3α,11β,17,20,21-Pentahydroxy-5β-pregnane tris(trimethylsilyl) ether; Tetrahydrocortisol Tris-TMS; THF Tris-TMS; (20R)-THF Tris(trimethylsilyl) Ether; Allotetrahydrocortisol TMS Derivative; 5β-Pregnane-3α,11β,17α,20α,21-pentol tris(trimethylsilyl) ether
EINECS	Contact for details

Quality Control

This high-purity reference material is produced under strict quality control protocols. Each batch is characterized using advanced analytical techniques including NMR, HPLC, and GC-MS to confirm identity and purity. A comprehensive Certificate of Analysis (COA) is provided, detailing batch-specific results for purity, residual solvents, and other critical parameters. Our quality system is designed to meet the exacting standards of pharmaceutical and clinical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (GC-MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Purity (GC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.