Description

Acrylic Acid n-Hydroxysuccinimide Ester is a highly reactive, bifunctional chemical linker that combines an acrylic ester group with an N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, covalent bonds between amine-containing molecules and surfaces that can undergo free-radical polymerization. It is a critical reagent for researchers and manufacturers in the bioconjugation and materials science fields who require precise and efficient crosslinking strategies. The product is supplied under strict quality control to ensure reliable performance in sensitive applications.

Application

• Bioconjugation and Protein Labeling: Used to covalently attach proteins, peptides, or antibodies to various surfaces (e.g., microarrays, biosensors, nanoparticles) via the amine-reactive NHS ester, while the acrylic group allows for subsequent copolymerization.
• Hydrogel Synthesis: Serves as a functional crosslinker in the preparation of polyacrylamide or polyethylene glycol (PEG) based hydrogels for drug delivery, tissue engineering, and 3D cell culture.
• Surface Modification and Immobilization: Enables the permanent functionalization of polymer, glass, or metal surfaces with bioactive molecules to create bioactive interfaces for diagnostic devices and lab-on-a-chip systems.
• Polymer Functionalization: Incorporated into polymer backbones to introduce reactive NHS ester sites for post-polymerization modification, creating smart materials with tailored properties.
• Affinity Chromatography Resin Preparation: Used to immobilize ligands onto chromatographic supports for the purification of biomolecules.
• Diagnostic Assay Development: Facilitates the stable attachment of capture antibodies or antigens to solid phases in ELISA and lateral flow assays.

Basic Information

Product Name	Acrylic Acid n-Hydroxysuccinimide Ester
CAS No.	56905-55-6
Molecular Formula	C7H7NO5
Molecular Weight	185.13 g/mol
Synonyms	Acryloyloxy succinimide; N-Acryloxysuccinimide; N-(Acryloyloxy)succinimide; Acrylic Acid N-Hydroxysuccinimide Ester; NHS-Acrylate; Acryloyl NHS ester; Succinimidyl acrylate; 2-Propenoic acid, 2,5-dioxo-1-pyrrolidinyl ester
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and manufacturing. Each batch is characterized to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing specific test results are available upon request. We adhere to a quality management system to guarantee batch-to-batch consistency and supply reliability for your critical processes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent hydrolysis of the active ester. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Melting Point	72 - 78 °C
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.