Description

Phthalyl-L-γ-Glutamyl-4-Nitroanilide is a specialized chromogenic substrate designed for enzymatic assays and biochemical research. This compound is critical for the precise detection and quantification of specific protease activity, offering high sensitivity and selectivity. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology and proteomics.

Application

• Protease Activity Assays: As a chromogenic substrate for the specific detection and kinetic analysis of γ-glutamyl transpeptidase (GGT) and related enzymes.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for liver function and other biomarker tests.
• Biochemical Research: Used in academic and industrial laboratories to study enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic enzyme inhibitors.
• Quality Control in Bioprocessing: Monitoring enzyme activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Phthalyl-L-γ-Glutamyl-4-Nitroanilide
CAS No.	56816-40-1
Molecular Formula	C₂₀H₁₈N₄O₇
Molecular Weight	426.38 g/mol
Synonyms	L-γ-Glutamyl-4-nitroanilide phthalyl derivative; Phthalyl-L-γ-Glutamyl-p-Nitroanilide; 4-Nitroaniline, N-[N-[1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl]-L-γ-glutamyl]-; GGT Substrate; γ-Glutamyltransferase Substrate; L-γ-Glutamyl-4-nitroanilide phthalyl compound; N-Phthaloyl-L-γ-glutamyl-4-nitroanilide
EINECS	Contact for details

Quality Control

Our Phthalyl-L-γ-Glutamyl-4-Nitroanilide is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, colorless solution in DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.