Description

m-Nitrotetrazolium Blue (CAS NO 56576-92-2) is a specialized tetrazolium salt widely recognized for its role as a redox indicator in biochemical assays. Its primary value lies in its ability to form a colored formazan product upon reduction, making it an essential tool for detecting cellular metabolic activity and enzymatic function. This compound is critically needed by researchers and quality control laboratories in the life sciences, pharmaceutical development, and diagnostic manufacturing sectors.

Application

• Biochemical Assay Reagent: Used as a key substrate in colorimetric assays to measure dehydrogenase enzyme activity, such as succinate dehydrogenase in mitochondrial studies.
• Cell Viability and Cytotoxicity Testing: Employed in MTT assay alternatives to quantify metabolically active cells, crucial for drug screening and toxicology research.
• Histochemical Staining: Applied in tissue sections to localize sites of enzymatic activity, particularly in neuroscience and pathology.
• Microbiology Research: Utilized to assess bacterial viability and metabolic function in environmental and clinical microbiology studies.
• Pharmaceutical Quality Control: Serves as a critical component in enzymatic test kits for raw material and finished product analysis.
• Plant Physiology Studies: Used to investigate respiratory activity and electron transport chain function in plant tissues.

Basic Information

Product Name	m-Nitrotetrazolium Blue
CAS No.	56576-92-2
Molecular Formula	C40H32Cl2N10O6
Molecular Weight	817.66 g/mol
Synonyms	m-Nitro Blue Tetrazolium; m-Nitro BT; m-NBT; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium] dichloride; Nitro Blue Tetrazolium Chloride (m-isomer); Tetrazolium Blue (m-nitro derivative); NBT (m-nitro); Nitrotetrazolium Blue Chloride
EINECS	Contact for details

Quality Control

Our m-Nitrotetrazolium Blue is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance characteristics, and loss on drying. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing batch-specific results to ensure consistency and traceability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Absorbance (λ max)	Conforms
Solubility (Test)	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.