Description

4-Methylumbelliferylb-D-Chitobioseheptaacetate is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase and related enzyme activities. This compound matters for researchers and industrial biochemists requiring precise, high-throughput enzymatic assays. It is primarily needed by the life sciences research sector, diagnostic kit manufacturers, and agrochemical companies developing chitinase-based biocontrol agents.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for the continuous assay of exo- and endo-chitinases (e.g., N-acetyl-β-D-glucosaminidase, chitobiosidase).
• Biochemical Research: Used in kinetic studies, enzyme characterization, and inhibitor screening for chitinolytic enzymes in academic and pharmaceutical research.
• Diagnostic Development: Employed in the development of diagnostic assays for fungal pathogens and conditions related to chitin metabolism.
• Agrochemical Screening: Utilized in high-throughput screening platforms to identify novel fungicides or biocontrol agents that target fungal cell wall integrity.
• Quality Control in Enzyme Production: Serves as a standard reagent for the quality control and activity validation of commercial chitinase preparations.

Basic Information

Product Name	4-Methylumbelliferylb-D-Chitobioseheptaacetate
CAS No.	55900-34-0
Molecular Formula	C41H49NO23
Molecular Weight	923.83 g/mol
Synonyms	4-MU-β-(GlcNAc)2-heptaacetate; 4-Methylumbelliferyl N,N'-diacetyl-β-D-chitobioside heptaacetate; 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside heptaacetate; 4-MU-Chitobiose heptaacetate; MUF-Chitobiose heptaacetate; 4-MU-(GlcNAc)2 heptaacetate; Fluorogenic chitobiose substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferylb-D-Chitobioseheptaacetate is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity, essential for reliable and reproducible assay results. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant guidelines for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility (in DMSO)	Clear, colorless solution at 10 mM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.