Description

n-Succinimidyl 6-Maleimidohexanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a thiol-reactive maleimide group. This compound is essential for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules, enabling advanced research and development. It is a critical tool for scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing stable, cleavable, or non-cleavable linkers that connect cytotoxic drugs to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: For creating defined protein complexes, fusion proteins, or attaching functional peptides to carrier proteins.
• Enzyme and Fluorescent Probe Labeling: Site-specific attachment of enzymes (e.g., HRP) or fluorescent dyes (e.g., Alexa Fluor, Cy dyes) to antibodies or other proteins for immunoassays and imaging.
• Surface Immobilization: Functionalizing gold surfaces, chips, or nanoparticles with biomolecules for biosensor development and microarray technology.
• Polymer Modification: Introducing thiol-reactive sites onto polymers or dendrimers for creating advanced drug delivery systems.
• Diagnostic Reagent Development: Manufacturing components for ELISA, lateral flow assays, and other clinical diagnostic kits.

Basic Information

Product Name	n-Succinimidyl 6-Maleimidohexanoate
CAS No.	55750-63-5
Molecular Formula	C15H18N2O6
Molecular Weight	322.31 g/mol
Synonyms	6-Maleimidocaproic Acid N-Hydroxysuccinimide Ester; SMCC; Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (common mis-assignment, but a distinct compound); NHS-PEG6-Maleimide (related, but not identical); N-Succinimidyl 6-maleimidohexanoate; EMCS (a similar compound with different spacer length); Maleimidocaproyl-N-hydroxysuccinimide; LC-SMCC (Long Chain SMCC).
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 6-Maleimidohexanoate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize hydrolysis. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%
Maleimide Content (Assay)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.