Description

n-Succinimidyl Maleimidoacetate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This dual functionality is critical for creating stable, site-specific conjugates between proteins, peptides, antibodies, and other biomolecules. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production, enabling precise control over molecular architecture.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing stable, cleavable, or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Labeling: For site-specific attachment of fluorophores, biotin, enzymes, or other probes to cysteine residues via the maleimide group, following initial amine coupling.
• Immobilization Chemistry: Functionalizing solid supports (e.g., beads, biosensor chips, plates) with maleimide groups for the oriented capture of thiol-containing ligands.
• Polymer-Protein Conjugation: Crafting PEGylated proteins or peptide-polymer hybrids to improve pharmacokinetics, solubility, and stability.
• Diagnostic Reagent Manufacturing: Conjugating enzymes (e.g., HRP, alkaline phosphatase) or other signal-generating molecules to antibodies or antigens for ELISA and lateral flow assays.
• Biomaterial Fabrication: Crosslinking hydrogels or modifying biomaterial surfaces to introduce specific bioactive motifs for tissue engineering and controlled release.

Basic Information

Product Name	n-Succinimidyl Maleimidoacetate
CAS No.	55750-61-3
Molecular Formula	C10H8N2O6
Molecular Weight	252.18 g/mol
Synonyms	Maleimidocetic Acid N-Hydroxysuccinimide Ester; NHS-Maleimide; SM(PEG)n Crosslinkers precursor; N-(Maleimidomethyl)succinimide Ester; N-Succinimidyl 3-Maleimidopropionate analog; SMA; Succinimidyl Maleimidoacetate; 4-Maleimidobutyric Acid N-Hydroxysuccinimide Ester (related compound)
EINECS	Contact for details

Quality Control

Our n-Succinimidyl Maleimidoacetate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, essential for sensitive bioconjugation applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our customers in preclinical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the maleimide group can degrade upon prolonged exposure to moisture or elevated temperatures. For long-term stability, store in aliquots under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.