Description

4-Methylumbelliferyl-2-Acetamido-4,6-*o- Benzylidene is a specialized fluorogenic substrate used for the detection and quantification of specific enzymatic activity. This compound matters for its high sensitivity and specificity, enabling precise biochemical and diagnostic assays. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics, high-throughput screening, and biomarker research.

Application

• Enzyme Substrate for Glycosidases: Specifically used as a chromogenic/fluorogenic substrate for enzymes like β-N-acetylhexosaminidase and related glycosyl hydrolases in biochemical research.
• Diagnostic Assay Development: A key component in the development of clinical diagnostic kits for lysosomal storage disorders (e.g., Tay-Sachs disease, Sandhoff disease).
• High-Throughput Screening (HTS): Employed in pharmaceutical research for screening potential enzyme inhibitors or activators in drug discovery pipelines.
• Biomarker Research: Used to measure specific enzyme activity levels in cell lysates, tissue samples, and biological fluids as a research biomarker.
• Quality Control in Biomanufacturing: Applied in monitoring enzyme activity during the production and purification of therapeutic proteins.
• Microbiology Studies: Utilized in research to study bacterial or fungal glycosidase activity for identification and characterization purposes.

Basic Information

Product Name	4-Methylumbelliferyl-2-Acetamido-4,6-*o- Benzylidene
CAS No.	55673-91-1
Molecular Formula	C24H23NO8
Molecular Weight	453.44 g/mol
Synonyms	4-Methylumbelliferyl 2-acetamido-4,6-O-benzylidene-2-deoxy-β-D-glucopyranoside; 4-MU-β-GlcNAc-benzylidene; 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide benzylidene derivative; Fluorogenic N-acetyl-β-D-glucosaminide substrate; 2'-(4-Methylumbelliferyl)-2-acetamido-4,6-O-benzylidene-2-deoxy-β-D-glucopyranoside; MUB-β-GlcNAc-benzylidene; HEX substrate (benzylidene); 4-MU-β-GlcNAc(Bn)
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-2-Acetamido-4,6-*o- Benzylidene is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance as a critical biochemical reagent. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Fluorogenic Activity	Conforms to functional standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.