Description

Endoproteinase Asp-N From Pseudomonas Fr Agi, 2 Ug is a highly specific proteolytic enzyme that cleaves peptide bonds on the N-terminal side of aspartic acid residues. This specificity is critical for precise protein sequencing, peptide mapping, and structural analysis in advanced biochemical research. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and proteomics sectors, where accurate protein characterization is paramount.

Application

• Protein Sequencing and Peptide Mapping: Used for the specific cleavage of proteins at aspartic acid residues to generate defined fragments for mass spectrometry analysis.
• Structural Biology Studies: Aids in determining protein structure and identifying post-translational modification sites by creating specific cleavage patterns.
• Biopharmaceutical Characterization: Critical for the analysis of monoclonal antibodies, recombinant proteins, and other therapeutic biologics to ensure product consistency and purity.
• Proteomics Research: Employed in bottom-up proteomics workflows for the digestion of complex protein mixtures prior to LC-MS/MS analysis.
• Disulfide Bond Analysis: Helps in mapping disulfide linkages within proteins due to its specific cleavage pattern, which can preserve these bonds.
• Quality Control Testing: Used in QC labs for the identity testing and impurity profiling of protein-based pharmaceuticals.

Basic Information

Product Name	Endoproteinase Asp-N From Pseudomonas Fr Agi, 2 Ug
CAS No.	55576-49-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Asp-N Endopeptidase; Asp-N Protease; Endoproteinase Asp-N; Pseudomonas fragi Asp-N protease; Aspartic Acid-N-specific Protease; Peptidase Asp-N; EC 3.4.24.33
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and analytical use. Each batch is tested for specific activity, purity, and the absence of non-specific proteolytic activities. Certificates of Analysis (COA) detailing assay results, storage buffer composition, and activity units are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize condensation and moisture uptake. For short-term use, aliquots may be stored at 2-8°C.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Cleaves N-terminal to Aspartic Acid residues
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 95%
Non-Specific Protease Activity	Not detected
Endotoxin Level	< 1.0 EU/µg (if applicable)
Storage Buffer	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.