Description

3Beta-Doxyl-5Alpha-Cholestane is a specialized spin-labeled sterol derivative, widely utilized as a molecular probe in biophysical and biochemical research. Its core value lies in its nitroxide radical (doxyl) group, which serves as a stable paramagnetic reporter for studying membrane dynamics, protein-lipid interactions, and structural analysis via techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. This high-purity compound is essential for researchers and developers in pharmaceutical R&D, advanced material science, and academic institutions focused on lipid bilayer characterization and spin-labeling methodologies.

Application

• As a spin-label probe for investigating the fluidity, phase transitions, and order parameters of lipid membranes and model bilayers using EPR spectroscopy.
• Studying the interaction and localization of sterols, peptides, and proteins within biological membranes.
• Use in structural biology for distance measurements via pulsed EPR techniques (e.g., DEER/PELDOR) to determine macromolecular conformations.
• Research on cholesterol trafficking, domain formation (lipid rafts), and membrane microdomain properties.
• Development and calibration of novel EPR methodologies and as a reference standard in spectroscopic analysis.
• Material science applications for studying the behavior of sterol-containing polymers or advanced synthetic membranes.

Basic Information

Product Name	3Beta-Doxyl-5Alpha-Cholestane
CAS No.	55569-61-4
Molecular Formula	C34H59NO2
Molecular Weight	513.84 g/mol
Synonyms	3β-Doxyl-5α-cholestane; Cholestane, 3-(1-oxyl-2,2-dimethyl-4-oxazolidinyl)-, (3β,5α)-; 3-DOXYL-5α-CHOLESTANE; 3-(1-Oxyl-2,2-dimethyl-4-oxazolidinyl)-5α-cholestane; 5α-Cholestan-3β-yloxy-4',4'-dimethyloxazolidine-N-oxyl; Spin-labeled cholesterol analog; 3β-DOXYL-cholestane; EPR spin probe
EINECS	Contact for details

Quality Control

Our 3Beta-Doxyl-5Alpha-Cholestane is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive spectroscopic applications. Each lot undergoes comprehensive analysis, including HPLC for purity verification, EPR spectroscopy to confirm radical integrity, and identity confirmation via IR and NMR. A detailed Certificate of Analysis (COA) documenting all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and ensure long-term stability of the nitroxide radical. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Off-white to pale yellow solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 97.0%
EPR Signal Intensity	Conforms to reference
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.