Description

Mutanolysin is a bacteriolytic enzyme, specifically a muramidase, derived from *Streptomyces globisporus* that selectively hydrolyzes the peptidoglycan layer of bacterial cell walls. This targeted activity makes it an indispensable tool for the controlled lysis of Gram-positive bacteria in research and industrial applications. It is primarily used by professionals in microbiology, pharmaceutical development, and biotechnology for cell wall analysis, protoplast preparation, and the extraction of intracellular components.

Application

• Microbiological Research: Essential for controlled lysis of Gram-positive bacteria (e.g., *Streptococcus*, *Staphylococcus*, *Listeria*) for genomic DNA, RNA, and protein extraction.
• Protoplast Generation: Used in combination with other enzymes to create cell wall-deficient protoplasts for genetic studies, transformation, and fusion experiments.
• Bacterial Cell Wall Analysis: A key reagent for structural studies of peptidoglycan, enabling the analysis of muropeptides and cross-linking patterns.
• Pharmaceutical Development: Employed in the development and quality control of antibiotics and vaccines that target bacterial cell wall synthesis.
• Diagnostic Assay Development: Utilized in the preparation of bacterial antigens or lysates for immunoassays and molecular diagnostic kits.
• Food Safety Testing: Applied in sample preparation protocols for the detection of pathogenic Gram-positive bacteria in food matrices.

Basic Information

Product Name	Mutanolysin
CAS No.	55466-22-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	~20,000 - 25,000 Da (Approx.)
Synonyms	Muranidase; Lysozyme (Streptomyces); N-Acetylmuramide glycanhydrolase; Peptidoglycan N-acetylmuramoylhydrolase; Streptomyces globisporus lytic enzyme; Mutanolysin from *Streptomyces globisporus*; Bacteriolytic enzyme; Cell wall lytic enzyme
EINECS	Contact for details

Quality Control

Our Mutanolysin is produced and tested under strict quality management systems to ensure high purity, specific activity, and lot-to-lot consistency suitable for research and industrial use. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as enzymatic activity, protein content, and purity. We adhere to relevant industry standards for biological reagents to guarantee reliable and reproducible performance in your applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). For short-term use, aliquots may be stored at 2-8°C. Avoid repeated freeze-thaw cycles to maintain optimal activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Protein Content (Biuret)	≥ 90%
Specific Activity	≥ 4,000 units/mg protein
Purity (SDS-PAGE)	Single major band
Solubility	Soluble in water or buffer
pH (1% solution)	6.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.