Description

6-Phosphofructokinase[Pyrophosphate] is a key regulatory enzyme in the glycolytic pathway, specifically catalyzing the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate using pyrophosphate as the phosphate donor. This enzyme is critical for research into carbohydrate metabolism, energy regulation, and metabolic disorders. It is primarily utilized by biochemical researchers, diagnostic kit manufacturers, and pharmaceutical companies engaged in metabolic pathway analysis and enzyme assay development.

Application

• Biochemical Research & Assay Development: Essential for in vitro studies of glycolysis, gluconeogenesis, and metabolic flux analysis.
• Diagnostic Reagent Manufacturing: Used as a core component in clinical diagnostic kits for measuring metabolites or enzyme activities related to metabolic health.
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) for identifying potential modulators of glycolytic activity as therapeutic targets for diseases like cancer or diabetes.
• Enzyme Kinetics Studies: Serves as a model enzyme for investigating allosteric regulation, catalytic mechanisms, and inhibitor interactions.
• Educational & Reference Standards: Used in academic and industrial laboratories for teaching advanced biochemistry and calibrating analytical equipment.

Basic Information

Product Name	6-Phosphofructokinase[Pyrophosphate]
CAS No.	55326-40-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pyrophosphate-dependent phosphofructokinase; PPi-PFK; PFK (PPi); EC 2.7.1.90; Fructose-6-phosphate 1-phosphotransferase (pyrophosphate); Phosphofructokinase (pyrophosphate); 6-Phosphofructokinase, pyrophosphate-dependent; ATP-D-fructose-6-phosphate 1-phosphotransferase (pyrophosphate)
EINECS	Contact for details

Quality Control

Our 6-Phosphofructokinase[Pyrophosphate] is produced and tested under stringent quality management systems. Each batch undergoes comprehensive analysis to ensure high specific activity, purity, and stability, meeting the rigorous demands of research and industrial applications. Certificates of Analysis (COA) detailing assay results, purity profiles, and stability data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported per batch
Stability	Retains ≥ 90% activity after 12 months at -20°C
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.