Description

4-Nitrophenyl B-D-Glucopyranoside-6-Phosphate is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its critical role in the detection and quantification of specific enzyme activities, particularly phosphatases and glycosidases. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science industries for biochemical analysis and assay development.

Application

• Enzyme Activity Assays: As a chromogenic substrate for alkaline phosphatase (ALP) and other phosphatases, producing a yellow-colored 4-nitrophenolate upon enzymatic cleavage for spectrophotometric detection.
• Diagnostic Kit Manufacturing: A key raw material in the production of clinical diagnostic kits for measuring enzyme levels in biological samples.
• Biochemical Research: Used in laboratory studies to investigate enzyme kinetics, inhibition, and metabolic pathways involving phosphate transfer.
• Pharmaceutical Quality Control: Employed in purity and potency testing of enzyme-based biopharmaceuticals during manufacturing.
• Cell Biology Studies: Useful as a substrate in histochemistry and cytochemistry to localize phosphatase activity within tissues and cells.
• Food Safety Testing: Potential application in assays designed to detect bacterial contamination or specific enzymatic activities in food products.

Basic Information

Product Name	4-Nitrophenyl B-D-Glucopyranoside-6-Phosphate
CAS No.	55196-70-8
Molecular Formula	C12H16NO12P
Molecular Weight	397.23 g/mol
Synonyms	p-Nitrophenyl β-D-glucopyranoside 6-phosphate; PNPG-6-P; 4-Nitrophenyl β-D-glucopyranoside 6-(dihydrogen phosphate); 6-O-Phosphono-β-D-glucopyranosyl 4-nitrobenzene; β-D-Glucopyranoside, 4-nitrophenyl, 6-(dihydrogen phosphate); p-Nitrophenyl-β-D-glucopyranoside-6-phosphate; 4-Nitrophenyl β-D-glucoside 6-phosphate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl B-D-Glucopyranoside-6-Phosphate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) documenting purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccant and under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.