Description

Nitrosylprotoheme is a coordination complex of heme with nitric oxide, a key intermediate in the study of nitric oxide biology and heme protein chemistry. This compound is essential for researchers and manufacturers investigating cellular signaling, vasodilation, and the biochemical pathways of nitrosative stress. It serves as a critical reference standard and biochemical tool for the pharmaceutical, biotechnology, and academic research sectors.

Application

• Biochemical Research: Used as a stable nitric oxide donor and a model compound for studying the interactions of nitric oxide with heme proteins like hemoglobin, myoglobin, and soluble guanylyl cyclase.
• Pharmaceutical Development: Acts as a reference standard and intermediate in the research and development of drugs targeting cardiovascular diseases and nitric oxide signaling pathways.
• Enzyme Mechanism Studies: Employed to probe the structure and function of nitric oxide synthases (NOS) and other heme-containing enzymes involved in gasotransmitter metabolism.
• Spectroscopic Calibration: Serves as a standard for UV-Vis, EPR, and other spectroscopic techniques due to its distinct spectral properties upon nitric oxide binding.
• Cell Signaling Research: Utilized in in vitro assays to study the role of nitrosyl-heme complexes in cellular communication, apoptosis, and immune response.

Basic Information

Product Name	Nitrosylprotoheme
CAS No.	54854-55-6
Molecular Formula	C34H32FeN4O4 (Heme b-NO complex)
Molecular Weight	616.49 g/mol (approx.)
Synonyms	Heme Nitrosyl Complex; Nitrosyl Heme; Nitrosylheme; Nitrosyl-Protoheme; Iron(II) Protoporphyrin IX Nitrosyl Complex; NO-Heme; Ferroheme Nitrosyl; Protoheme-NO
EINECS	Contact for details

Quality Control

Our Nitrosylprotoheme is produced and handled under controlled conditions to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes rigorous analytical testing, including spectroscopic identification and purity assessment. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and relevant spectral data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and degradation. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	Dark brown to black solid
Identification (IR)	Conforms to reference spectrum
Identification (UV-Vis)	Characteristic Soret and Q-band absorption
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.