Description

2'-Deoxyadenosine-5'-Triphosphate, Trisodium Salt is a high-purity nucleotide essential for enzymatic and biochemical research. This compound serves as a critical substrate for DNA polymerases, reverse transcriptases, and other enzymes involved in nucleic acid synthesis and manipulation. It is a fundamental reagent for molecular biology applications, including PCR, DNA sequencing, site-directed mutagenesis, and cDNA synthesis, making it indispensable for pharmaceutical R&D, diagnostic development, and academic research.

Application

• Polymerase Chain Reaction (PCR): Acts as one of the four essential dNTP building blocks for DNA amplification.
• DNA Sequencing: Used as a substrate in Sanger (dideoxy) sequencing and next-generation sequencing (NGS) workflows.
• cDNA Synthesis: Provides the necessary dATP for reverse transcription of RNA into complementary DNA.
• Site-Directed Mutagenesis: Essential for in vitro DNA synthesis and error-prone PCR techniques.
• Enzyme Kinetics & Assays: Used to study the activity and inhibition of DNA-dependent enzymes.
• DNA Labeling & Probe Synthesis: Can be used in reactions incorporating labeled nucleotides for detection.
• Biochemical Research: Fundamental for studies on DNA replication, repair, and recombination mechanisms.
• Diagnostic Kit Manufacturing: A key raw material in the production of molecular diagnostic tests and kits.

Basic Information

Product Name	2'-Deoxyadenosine-5'-Triphosphate, Trisodium Salt
CAS No.	54680-12-5
Molecular Formula	C10H14N5O12P3Na3
Molecular Weight	553.1 g/mol (anhydrous basis)
Synonyms	dATP Trisodium Salt; 2'-Deoxyadenosine 5'-triphosphate trisodium salt; Deoxyadenosine triphosphate trisodium salt; dATP Na3; Adenosine 5'-(tetrahydrogen triphosphate), 2'-deoxy-, trisodium salt; 5'-dATP Trisodium; Deoxyadenosine-5'-triphosphate trisodium; (2R,3S,5R)-5-(6-Amino-9H-purin-9-yl)-2-(hydroxymethyl)tetrahydrofuran-3-yl trihydrogen triphosphate trisodium salt
EINECS	Contact for details

Quality Control

Our 2'-Deoxyadenosine-5'-Triphosphate, Trisodium Salt is manufactured under strict quality systems to ensure consistency and reliability for sensitive research applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity assessment and identity confirmation. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles. The product is suitable for use in GMP-compliant manufacturing processes for diagnostics and pharmaceuticals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This material is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (UV)	Conforms
Purity (HPLC)	≥ 95%
pH (1% Solution)	6.0 - 8.0
Water Content (KF)	≤ 5.0%
Endotoxin	< 10 EU/mg
Biological Activity	Suitable for enzymatic reactions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.