Description

4-Nitrophenyl Dihydrogen Phosphate, Sodium Salt is a high-purity biochemical reagent widely used as a chromogenic substrate for enzyme activity assays. Its core value lies in its ability to produce a measurable colorimetric signal upon enzymatic hydrolysis, enabling precise quantification of enzyme kinetics. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors requiring reliable and sensitive assay components.

Application

• Enzyme Substrate for Phosphatase Assays: Primary substrate for alkaline phosphatase (ALP) and acid phosphatase activity determination in biochemical research and clinical diagnostics.
• Diagnostic Kit Manufacturing: A key component in the formulation of commercial diagnostic kits for measuring phosphatase levels in biological samples.
• Drug Discovery & High-Throughput Screening (HTS): Used in enzymatic assays to identify and characterize potential therapeutic inhibitors or activators of phosphatase enzymes.
• Biochemical Research: Employed in studying enzyme kinetics, mechanism of action, and purification processes of various phosphatases.
• Food & Environmental Analysis: Applied in assays to detect phosphatase activity as an indicator of pasteurization efficacy or environmental contamination.
• Quality Control in Biotechnology: Used to standardize and validate the activity of enzyme preparations and recombinant proteins.

Basic Information

Product Name	4-Nitrophenyl Dihydrogen Phosphate, Sodium Salt
CAS No.	54306-27-3
Molecular Formula	C6H5NNa2O6P
Molecular Weight	263.07 g/mol
Synonyms	p-Nitrophenyl Phosphate, Disodium Salt; PNPP; 4-NPP; Disodium 4-Nitrophenyl Phosphate; Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt; p-Nitrophenyl Phosphate Disodium Salt Hexahydrate; ALP Substrate; Acid Phosphatase Substrate.
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Dihydrogen Phosphate, Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
UV-Vis Absorbance	Conforms
Water Content (KF)	≤ 2.0%
Solubility	Clear, colorless solution in water
Enzymatic Suitability	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.