Description

6-(2,2,6,6--Tetramethylpiperidine-1-Oxyl)-Adenosine Monophosphate is a stable nitroxide radical-labeled derivative of adenosine monophosphate (AMP), a fundamental nucleotide. This compound is a critical spin-labeling reagent, enabling advanced research in structural biology and biophysics through techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is essential for scientists and R&D teams in pharmaceutical development, biochemistry, and life sciences who require precise molecular probes to study protein dynamics, nucleic acid interactions, and conformational changes.

Application

• EPR/ESR Spectroscopy Probe: Acts as a site-directed spin label for studying macromolecular structure, dynamics, and distances in proteins and nucleic acids.
• Biophysical Research: Used to investigate enzyme mechanisms, protein folding, and ligand-binding interactions by introducing a stable radical reporter group.
• Structural Biology: Aids in the determination of biomolecular structures and conformational changes through distance measurements via pulsed EPR techniques like DEER (Double Electron-Electron Resonance).
• Nucleotide Analogue Studies: Serves as a modified nucleotide to probe the function and interactions of ATP-binding proteins, kinases, and other nucleotide-dependent enzymes.
• Oxidative Stress & Redox Biology: Can be utilized as a radical scavenger or a redox-sensitive probe in cellular and biochemical assays.
• Pharmaceutical R&D: Employed in drug discovery to characterize target engagement and the molecular mechanisms of potential therapeutics.

Basic Information

Product Name	6-(2,2,6,6--Tetramethylpiperidine-1-Oxyl)-Adenosine Monophosphate
CAS No.	54187-54-1
Molecular Formula	C19H31N6O8P
Molecular Weight	502.46 g/mol
Synonyms	6-TEMPO-Adenosine Monophosphate; TEMPO-AMP; 6-(2,2,6,6-Tetramethyl-1-oxyl-4-piperidinyl)adenosine 5'-Monophosphate; Adenosine 5'-Monophosphate, 6-(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)-; AMP-TEMPO; Spin-labeled AMP; 6-(2,2,6,6-Tetramethylpiperidin-1-yloxyl)adenosine 5'-Monophosphate; Nitroxyl-AMP
EINECS	Contact for details

Quality Control

Our 6-(2,2,6,6--Tetramethylpiperidine-1-Oxyl)-Adenosine Monophosphate is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications such as assay, radical content, and impurity profile.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is easily oxidized and light-sensitive. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95%
Assay (UV-Vis)	≥ 90% (by radical content)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.