Description

Glycine-p-Nitroanilide is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of protease activity, particularly for enzymes like trypsin and thrombin, due to its specific cleavage properties. It serves as an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors, where precise enzymatic measurement is paramount.

Application

• Protease Activity Assays: Primary substrate for the colorimetric determination of trypsin, thrombin, and other serine proteases.
• Diagnostic Kit Manufacturing: Used as a key component in the production of clinical diagnostic kits for measuring enzymatic levels in biological samples.
• Biochemical Research: Employed in academic and industrial research to study enzyme kinetics, inhibition, and mechanism of action.
• Pharmaceutical Quality Control: Utilized in QC labs to test the activity and purity of enzyme-based pharmaceutical products.
• Food Science Analysis: Applied in methods to assess protease activity in food products and processing enzymes.

Basic Information

Item	Details
Product Name	Glycine-p-Nitroanilide
CAS No.	53987-32-9
Molecular Formula	C8H9N3O4
Molecular Weight	211.18 g/mol
Synonyms	H-Gly-pNA; Glycine 4-nitroanilide; N-(4-Nitrophenyl)glycinamide; Gly-pNA; 4-Nitroanilide of glycine; Glycine p-nitroanilide; p-Nitroanilide of glycine; Substrate for trypsin
EINECS	Contact for details

Quality Control

Our Glycine-p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the high standards required for sensitive biochemical applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and consistent performance for your critical assays.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.