Description

Estrone-2,4,16,16-D4 is a stable isotope-labeled analog of the natural estrogen estrone, specifically deuterated at the 2, 4, and 16 positions. This compound is critically important as an internal standard in quantitative mass spectrometry, enabling highly accurate and precise measurement of endogenous estrone levels in complex biological matrices. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, clinical research, and endocrinology fields, where reliable analytical data is paramount.

Application

• Mass Spectrometry Internal Standard: Primary use as a deuterated internal standard for the accurate quantification of estrone via LC-MS/MS and GC-MS methods.
• Clinical Diagnostics & Research: Used in the development and validation of assays for hormone level testing, metabolic disorder studies, and endocrine research.
• Pharmaceutical R&D: Employed in pharmacokinetic and pharmacodynamic studies of estrogen-based therapeutics and hormone replacement therapies.
• Doping Control Analysis: Critical for anti-doping laboratories to detect and confirm the abuse of endogenous steroids in athletes.
• Forensic Toxicology: Applied in the precise measurement of steroid hormones in forensic casework and post-mortem analyses.
• Environmental Analysis: Used as a reference standard in tracing and quantifying natural and synthetic estrogens in environmental samples.

Basic Information

Item	Detail
Product Name	Estrone-2,4,16,16-D4
CAS No.	53866-34-5
Molecular Formula	C18H18D4O2
Molecular Weight	274.39 g/mol
Synonyms	Estrone-d4; [2,4,16,16-2H4]-Estrone; 3-Hydroxy-1,3,5(10)-estratrien-17-one-2,4,16,16-d4; Deuterated Estrone; 1,3,5(10)-Estratrien-3-ol-17-one-2,4,16,16-d4; Estra-1,3,5(10)-trien-3-ol-17-one-2,4,16,16-d4; E1-d4
EINECS	Contact for details

Quality Control

Our Estrone-2,4,16,16-D4 is manufactured under strict quality systems to ensure the high chemical purity and isotopic enrichment required for analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and mass spectrometry, to confirm identity, purity, and deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.