Description

p-Nitrophenyl-n-Acetyl-1-Thio-B-D-*Gluco Saminide is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of specific glycosidase enzymes, such as N-acetyl-β-D-glucosaminidase (NAGase), which serve as important biomarkers. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for biochemical research, enzyme characterization, and diagnostic kit development.

Application

• Enzymatic Assay Substrate: Primary chromogenic substrate for the spectrophotometric detection of N-acetyl-β-D-glucosaminidase (NAGase) activity.
• Diagnostic Reagent Manufacturing: Key raw material in the production of diagnostic kits for clinical analysis, including the detection of lysosomal storage disorders and renal tubular damage.
• Biochemical Research: Used in academic and industrial research to study enzyme kinetics, inhibition, and the role of glycosidases in biological pathways.
• Pharmaceutical Quality Control: Employed in QC labs for testing enzyme-based pharmaceuticals and monitoring fermentation processes.
• Microbiology: Potential use in microbial identification systems that rely on specific glycosidase profiles.

Basic Information

Product Name	p-Nitrophenyl-n-Acetyl-1-Thio-B-D-*Gluco Saminide
CAS No.	53735-94-7
Molecular Formula	C14H18N2O7S
Molecular Weight	358.37 g/mol
Synonyms	4-Nitrophenyl 2-acetamido-2-deoxy-1-thio-β-D-glucopyranoside; p-Nitrophenyl N-acetyl-1-thio-β-D-glucosaminide; PNP-NAG; 1-Thio-β-D-glucosaminide, 4-nitrophenyl N-acetyl-; NAGase Substrate; 4-Nitrophenyl 2-acetamido-2-deoxy-1-thio-β-D-glucoside; 2-Acetamido-2-deoxy-1-thio-β-D-glucopyranoside 4-nitrophenyl ester
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl-n-Acetyl-1-Thio-B-D-*Gluco Saminide is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.