Description

2'-O,3,3'-O,5'-o-Tetramethyluridine CAS NO 53657-37-7 is a chemically modified nucleoside derivative, specifically a tetramethylated analog of uridine. This compound is of significant value as a key intermediate and building block in advanced oligonucleotide synthesis, particularly for the development of therapeutic nucleic acids. It is primarily needed by research institutions, pharmaceutical developers, and fine chemical manufacturers working on antisense oligonucleotides, siRNA, and other novel RNA-based therapeutics where modified backbones are essential for stability and efficacy.

Application

• Oligonucleotide Synthesis: A critical protected intermediate for the solid-phase and solution-phase synthesis of modified RNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) designed for targeted gene silencing.
• siRNA and miRNA Therapeutics: Serves as a building block to introduce backbone modifications that enhance nuclease resistance and pharmacokinetic properties of small interfering RNA (siRNA) and microRNA (miRNA) mimics.
• Pharmaceutical Research: Employed in medicinal chemistry for the discovery and optimization of novel nucleic acid-based drugs.
• Diagnostic Probe Development: Utilized in creating stable, labeled nucleic acid probes for diagnostic assays and genomic research.
• Bioconjugation Chemistry: Acts as a starting material for further functionalization and conjugation with other molecules (e.g., dyes, targeting ligands).

Basic Information

Product Name	2'-O,3,3'-O,5'-o-Tetramethyluridine
CAS No.	53657-37-7
Molecular Formula	C14H20N2O7
Molecular Weight	328.32 g/mol
Synonyms	2'-O,3,3'-O,5'-O-Tetramethyluridine; 1-[(2R,3R,4R,5R)-3,4-Bis(methoxymethyl)-5-(methoxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; 2',3',5'-Tri-O-methyluridine 3'-O-methyl ether; Uridine, 2',3,3',5'-tetramethyl-; Tetramethyluridine; Protected Uridine Derivative
EINECS	Contact for details

Quality Control

Our 2'-O,3,3'-O,5'-o-Tetramethyluridine is manufactured under strict quality systems suitable for pharmaceutical intermediate applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure compliance with stringent specifications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and support your regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.