Description

4-Methylumbelliferyl β-D-N,N',N',N''-Tetraacetylchitotetraoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase and lysozyme activity. This compound matters because it provides a highly specific and convenient tool for enzymatic research, enabling real-time kinetic analysis through the release of the fluorescent 4-methylumbelliferone upon cleavage. It is primarily needed by researchers and quality control laboratories in the biotechnology, pharmaceutical, and agricultural sectors for studying enzyme kinetics, inhibitor screening, and pathogen detection.

Application

• Chitinase Activity Assays: A standard fluorogenic substrate for measuring endochitinase and exochitinase activity in vitro.
• Lysozyme Research: Used to study the enzymatic activity and kinetics of lysozyme, which can hydrolyze chitotetraose derivatives.
• Fungal and Insect Research: Essential for investigating chitin metabolism in fungi, insects, and nematodes, aiding in the development of antifungal and insecticidal agents.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify novel chitinase inhibitors as potential therapeutic candidates.
• Plant Pathology Studies: Used to assay chitinase activity in plants as part of defense mechanism research against fungal pathogens.
• Biomarker Development: Facilitates the development of diagnostic assays for diseases where chitinase levels serve as a biomarker.
• Enzyme Purification Monitoring: Acts as a specific substrate to monitor chitinase activity during protein purification processes.
• Educational and Reference Standard: Serves as a critical reagent in academic research and as a certified reference material for method validation.

Basic Information

Product Name	4-Methylumbelliferyl β-D-N,N',N',N''-Tetraacetylchitotetraoside
CAS No.	53643-14-4
Molecular Formula	C47H62N4O26
Molecular Weight	1083.01 g/mol
Synonyms	4-MU-(GlcNAc)4; 4-Methylumbelliferyl N,N',N'',N'''-tetraacetylchitotetraoside; 4-Methylumbelliferyl β-D-tetra-N-acetylchitotetraoside; MUF-(GlcNAc)4; 4-MU-β-D-(GlcNAc)4; Fluorogenic chitotetraoside substrate; Chitinase substrate IV
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl β-D-N,N',N',N''-Tetraacetylchitotetraoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive enzymatic research. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and reliable performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.