Description

4-Methylumbelliferyl β-D-N,N'-Diacetyl-Chitobioside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase and lysozyme enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays in biochemical and pharmaceutical research. It is primarily utilized in life science research, diagnostic kit development, and industrial enzyme characterization for applications ranging from antifungal drug discovery to biomass conversion studies.

Application

• Chitinase Activity Assays: A standard fluorogenic substrate for measuring endo- and exo-chitinase activity in research and diagnostic applications.
• Lysozyme Detection: Used in sensitive fluorometric assays to quantify lysozyme activity, relevant in immunology and clinical studies.
• Antifungal Drug Screening: Enables high-throughput screening for compounds that inhibit fungal chitin synthesis, a key target for antifungal agents.
• Biomass & Biofuel Research: Employed in studies of chitin degradation for waste valorization and biofuel production processes.
• Plant Pathology & Defense Studies: Used to analyze chitinase expression in plants as a defense mechanism against fungal pathogens.
• Diagnostic Kit Manufacturing: A key component in the formulation of commercial diagnostic kits for enzyme deficiency disorders or infection markers.
• Quality Control in Enzyme Production: Serves as a critical reagent for standardizing and validating batches of purified chitinase enzymes.
• Marine & Environmental Biology: Applied in studies of chitinolytic organisms and chitin recycling in marine and soil ecosystems.

Basic Information

Product Name	4-Methylumbelliferyl β-D-N,N'-Diacetyl-Chitobioside
CAS No.	53643-12-2
Molecular Formula	C26H32N2O13
Molecular Weight	580.54 g/mol
Synonyms	4-MU-(GlcNAc)2; 4-Methylumbelliferyl N,N'-diacetyl-β-D-chitobioside; 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside; MUF-(GlcNAc)2; 4-MU-β-D-(GlcNAc)2; Fluorogenic chitobioside substrate; 7-Hydroxy-4-methylcoumarin N,N'-diacetyl-β-D-chitobioside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl β-D-N,N'-Diacetyl-Chitobioside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality guidelines to support our global customer base in pharmaceutical R&D and life sciences.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.