Description

Fluoresceinamine Hydrochloride Isomer 1 is a high-purity, reactive derivative of fluorescein, specifically the amine-functionalized isomer 1 in its hydrochloride salt form. This compound is a critical building block for the synthesis of fluorescent probes, labels, and conjugates due to its excellent fluorescence properties and amine reactivity. It is essential for researchers and manufacturers in the life sciences, diagnostics, and analytical chemistry sectors who require precise and reliable fluorescent tagging reagents for biomolecule analysis, immunoassays, and cellular imaging.

Application

• Fluorescent Probe Synthesis: A key precursor for creating custom fluorescent labels and sensors targeting specific biomolecules.
• Bioconjugation & Labeling: Used to covalently attach fluorescent tags to antibodies, proteins, peptides, and oligonucleotides for detection and visualization.
• Diagnostic Assays: Employed in the development of fluorescent immunoassays (FLISA), lateral flow tests, and other in-vitro diagnostic (IVD) components.
• Cellular & Molecular Imaging: Facilitates the study of cellular processes, receptor targeting, and intracellular trafficking when conjugated to targeting ligands.
• Material Science & Sensors: Incorporated into polymers, nanoparticles, and solid supports to create fluorescent sensing materials and smart surfaces.
• Chromatography & Electrophoresis: Serves as a derivatization agent for the sensitive detection of amines, amino acids, and other analytes in separation sciences.

Basic Information

Product Name	Fluoresceinamine Hydrochloride Isomer 1
CAS No.	53360-53-5
Molecular Formula	C20H14ClNO5 • HCl
Molecular Weight	420.25 g/mol (as hydrochloride salt)
Synonyms	5-Aminofluorescein Hydrochloride (Isomer I); 6-Aminofluorescein Hydrochloride; Fluorescein-5-amine Hydrochloride; 3',6'-Dihydroxy-5-amino-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one Hydrochloride; 5-AF HCl; 5-FAMamine HCl; Reactive Fluorescein Derivative; Fluorescent Amine Labeling Reagent
EINECS	Contact for details

Quality Control

Our Fluoresceinamine Hydrochloride Isomer 1 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed lot-specific data are provided to guarantee traceability, consistency, and performance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Isomer 1)
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm
Fluorescence	Conforms to reference (λex/λem ~492/518 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.