Description

n-Hydroxysuccinimidyl-4-Azidobenzoyl Glycine is a heterobifunctional crosslinking reagent that combines a photoactivatable aryl azide group with an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound matters for its ability to facilitate the site-specific conjugation of biomolecules under mild, controllable conditions, primarily through UV light activation. It is essential for researchers and manufacturers in bioconjugation, proteomics, and materials surface functionalization who require precise and stable linkages between diverse molecular entities.

Application

• Photocrosslinking in Proteomics: For mapping protein-protein interactions and identifying binding sites by capturing transient complexes upon UV irradiation.
• Surface Immobilization: Functionalizing chips, beads, or biosensor surfaces with peptides, antibodies, or other ligands for assay development.
• Bioconjugate Synthesis: Creating stable conjugates between proteins, antibodies, or enzymes and other molecules like dyes, drugs, or polymers.
• Hydrogel Formation: Serving as a crosslinker in the development of photo-patternable biomaterials for tissue engineering and drug delivery.
• Affinity Labeling: Tagging specific receptors or active sites in biological systems for purification or analytical studies.
• Polymer Modification: Introducing bioactive groups onto synthetic polymer backbones to create functional hybrid materials.

Basic Information

Product Name	n-Hydroxysuccinimidyl-4-Azidobenzoyl Glycine
CAS No.	53122-84-2
Molecular Formula	C15H14N4O6
Molecular Weight	346.30 g/mol
Synonyms	4-Azidobenzoyl Glycine NHS Ester; NHS-4-Azidobenzoyl Glycine; N-Succinimidyl 4-Azidobenzoylglycinate; SAG; Glycine, N-(4-azidobenzoyl)-, N-succinimidyl ester; Heterobifunctional Crosslinker, NHS-Azide; 4-Azidobenzoic Acid Glycine NHS Ester; N-(4-Azidobenzoyl)glycine N-Hydroxysuccinimide Ester
EINECS	Contact for details

Quality Control

Our n-Hydroxysuccinimidyl-4-Azidobenzoyl Glycine is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and FT-IR for functional group verification. Certificates of Analysis (COA) detailing all specifications are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and strictly light-sensitive (protect from all light exposure). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.