Description

3-Nitrophenyl-α-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of α-galactosidase activity, a key enzyme in biochemical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for high-sensitivity assays.

Application

• Enzymatic Substrate for α-Galactosidase: Serves as a specific chromogenic substrate in biochemical assays to measure α-galactosidase enzyme activity.
• Diagnostic Kit Manufacturing: A key raw material in the production of diagnostic kits for genetic disorders like Fabry disease, which involves α-galactosidase A deficiency.
• Biochemical Research: Used in laboratory research to study enzyme kinetics, inhibition, and function in various biological pathways.
• Microbiology and Cell Biology: Employed in assays to detect reporter gene expression (e.g., lacZ gene analogs) in microbial and mammalian cell systems.
• Quality Control in Biopharma: Applied in the quality control testing of enzyme preparations and recombinant proteins.
• Food & Beverage Analysis: Potential use in enzymatic methods for food analysis where specific glycosidase activities need to be monitored.

Basic Information

Product Name	3-Nitrophenyl-α-D-Galactopyranoside
CAS No.	52571-71-8
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	3-Nitrophenyl α-D-galactopyranoside; p-Nitrophenyl α-D-galactopyranoside; 4-Nitrophenyl α-D-galactopyranoside; α-D-Galactopyranoside, 3-nitrophenyl; α-Galactosidase substrate; PNPGal (α); 3-NPG; 3-Nitrophenyl-α-D-galactoside; α-PNPG
EINECS	Contact for details

Quality Control

Our 3-Nitrophenyl-α-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. The product is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.