Description

Thymidine, [Methyl-3H] is a tritium-labeled nucleoside derivative essential for advanced biochemical research and diagnostic development. This radiolabeled compound provides a critical tool for tracing and quantifying DNA synthesis, metabolism, and repair mechanisms with high sensitivity. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic manufacturers engaged in molecular biology, oncology research, and radiopharmaceutical precursor synthesis. The product is supplied to meet the stringent requirements of isotopic purity and specific activity necessary for autoradiography and liquid scintillation counting applications.

Application

• DNA Synthesis & Cell Proliferation Studies: Used as a radiolabeled tracer to measure rates of DNA replication and cell proliferation in vitro and in vivo.
• Oncology & Cancer Research: Employed in assays to evaluate the efficacy of chemotherapeutic agents and radiation therapy on tumor cell growth.
• Molecular Biology & Biochemistry: Serves as a precursor in enzymatic reactions and for studying nucleotide metabolism pathways.
• Autoradiography: Incorporated into nucleic acids to visualize and localize DNA synthesis in tissues and cell cultures.
• Radiopharmaceutical Development: Acts as a key intermediate or research tool in the development of novel diagnostic imaging agents.
• Toxicology & Genotoxicity Testing: Used in standard assays (e.g., unscheduled DNA synthesis tests) to assess the DNA-damaging potential of chemicals.
• Virology Research: Applied in studies of viral replication mechanisms that involve host cell DNA synthesis.
• Quality Control for Diagnostic Kits: Utilized as a reference standard in the production and validation of diagnostic assays.

Basic Information

Product Name	Thymidine, [Methyl-3H]
CAS No.	52497-68-4
Molecular Formula	C10H14N2O5 (³H labeled)
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, [Methyl-³H]; [³H]Thymidine; Thymidine, methyl-³H; Tritiated Thymidine; 2'-Deoxy[³H]thymidine; [³H]dT; 5-Methyl-2'-deoxy[³H]uridine; 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyl[³H]uracil
EINECS	Contact for details

Quality Control

Every batch of Thymidine, [Methyl-3H] is manufactured and tested under controlled conditions to ensure exceptional isotopic and chemical purity. Our quality assurance protocol includes rigorous analysis for specific activity, radiochemical purity, and chemical identity. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results from HPLC, radio-HPLC, and spectroscopic methods. We adhere to relevant Good Manufacturing Practice (GMP) principles for radioactive materials to guarantee consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in the original solvent as supplied. For long-term storage, maintain under inert atmosphere to prevent oxidation. This product is light-sensitive and should be handled in appropriate laboratory conditions to preserve stability and specific activity.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	Contact for details (Ci/mmol range)
Radiochemical Purity (Radio-HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (specific ratio available on COA)
Concentration	1 mCi/mL (Nominal)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.