Description

4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt is a high-purity chromogenic substrate widely used in enzymatic assays. This compound is critical for the sensitive and quantitative detection of alkaline phosphatase activity. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors, where reliable and reproducible results are paramount.

Application

• Primary Substrate for Alkaline Phosphatase (ALP) Assays: Used in ELISA, immunoblotting (western blot), and other colorimetric detection systems where ALP is the reporter enzyme.
• Clinical Diagnostics: A key component in diagnostic kits for measuring ALP levels in serum, a biomarker for liver and bone disorders.
• Molecular Biology Research: Employed in nucleic acid hybridization detection methods, such as non-radioactive labeling and detection of DNA/RNA probes.
• Pharmaceutical Quality Control: Used in potency testing and impurity profiling of biopharmaceuticals that utilize ALP-conjugated antibodies or other proteins.
• Food Safety Testing: Applied in certain enzyme-linked immunosorbent assays (ELISAs) for the detection of pathogens or toxins in food samples.
• High-Throughput Screening (HTS): Suitable for automated screening platforms in drug discovery to identify enzyme inhibitors or activators.

Basic Information

Item	Details
Product Name	4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt
CAS No.	52483-84-8
Molecular Formula	C18H34N3O6P
Molecular Weight	419.46 g/mol
Synonyms	p-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt; PNPP Bis(Cyclohexylammonium) Salt; 4-NPP Bis(Cyclohexylammonium) Salt; Phosphoric Acid 4-Nitrophenyl Ester Bis(Cyclohexylammonium) Salt; Disodium p-Nitrophenyl Phosphate Alternative; ALP Substrate; Alkaline Phosphatase Substrate; Chromogenic Phosphatase Substrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate Bis(Cyclohexylammonium) Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive diagnostic and research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (Enzymatic)	Suitable for use
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.