Description

p-Nitrophenyl Phosphate Trisbuffer Salt is a high-purity chromogenic substrate widely used in enzymatic assays. Its primary value lies in its role as a substrate for alkaline phosphatase (ALP), where enzymatic hydrolysis produces the yellow-colored p-nitrophenol, enabling sensitive colorimetric detection. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing industries, supporting critical applications in drug discovery, immunoassays, and molecular biology.

Application

• Alkaline Phosphatase (ALP) Activity Assays: Standard substrate for quantifying ALP activity in clinical diagnostics and biochemical research.
• ELISA (Enzyme-Linked Immunosorbent Assay): Key component in colorimetric detection systems for antibody-antigen interactions.
• Diagnostic Kit Manufacturing: Used as a critical raw material in the production of commercial clinical diagnostic test kits.
• Molecular Biology Research: Employed in nucleic acid detection protocols and other enzyme-linked detection methods.
• Pharmaceutical Quality Control: Utilized in potency testing and enzymatic activity assays for drug development and batch release.
• Cell Signaling & Pathway Analysis: Applied in studies involving phosphatase activity as a cellular signal transducer.

Basic Information

Product Name	p-Nitrophenyl Phosphate Trisbuffer Salt
CAS No.	52435-04-8
Molecular Formula	C9H14NO10P
Molecular Weight	327.18 g/mol
Synonyms	PNPP Tris Salt; 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate (Tris-buffered); pNPP; Phosphoric Acid 4-Nitrophenyl Ester Tris Salt; ALP Substrate; Alkaline Phosphatase Substrate; 4-NPP Tris Salt; Diagnostic Grade PNPP
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl Phosphate Trisbuffer Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive diagnostic applications. Each lot is tested to meet stringent specifications for purity, identity, and functional performance. Certificates of Analysis (COA) detailing assay, impurity profile, and solution clarity are provided with every shipment to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the container to reach room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Solution Clarity (1% in buffer)	Clear, colorless solution
Water Content (KF)	≤ 2.0%
Free p-Nitrophenol (HPLC)	≤ 0.5%
Functional Test (ALP Activity)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.