Description

Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex is a specialized weak anion exchange chromatography medium derived from cross-linked dextran. This product matters for its ability to separate and purify biomolecules, such as proteins, enzymes, and nucleic acids, based on differences in their net surface charge. It is an essential tool for researchers and process engineers in the biotechnology, pharmaceutical, and life science industries who require high-resolution purification under mild conditions.

Application

• Biomolecule Purification: Isolation and purification of proteins, peptides, enzymes, and antibodies from complex biological mixtures.
• Nucleic Acid Separation: Fractionation of RNA, DNA, and oligonucleotides in molecular biology workflows.
• Process Development: Used in downstream process development and optimization for biopharmaceutical manufacturing.
• Laboratory-Scale Chromatography: A key medium for analytical and preparative column chromatography in research laboratories.
• Enzyme Immobilization: Serving as a support matrix for the immobilization of enzymes for biocatalysis applications.
• Desalting and Buffer Exchange: Facilitates the removal of salts and exchange of buffers in protein solutions.

Basic Information

Product Name	Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex
CAS No.	52219-08-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DEAE-Sephadex A-25; DEAE-Sephadex A-50; Diethylaminoethyl-Sephadex; 2-(Diethylamino)ethyl-Sephadex; Diethyl(2-hydroxypropyl)aminoethyl ether of Sephadex; QAE-Sephadex (related); Anion exchanger based on Sephadex; Weak base anion exchange resin
EINECS	Contact for details

Quality Control

Our Diethyl[2-Hydroxypropyl]Aminoethyl Sephadex is manufactured under a strict quality management system. Each batch undergoes rigorous testing for critical parameters including ionic capacity, particle size distribution, swelling characteristics, and functional group integrity to ensure consistent chromatographic performance. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed when not in use to prevent moisture uptake, which can affect swelling and performance. Avoid exposure to extreme temperatures and humidity.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Ionic Capacity	Contact for details
Particle Size	Contact for details
Swelling Volume	Contact for details
Maximum Operating Pressure	Contact for details
pH Stability	Contact for details
Exclusion Limit	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.