Description

D(-)-Amethopterin is the enantiomerically pure form of the antifolate compound methotrexate, a critical agent in biochemical and pharmaceutical research. This specific stereoisomer is essential for studying structure-activity relationships, receptor binding specificity, and metabolic pathways with high precision. It is primarily utilized by research institutions, pharmaceutical R&D departments, and advanced chemical synthesis facilities focused on oncology, immunology, and enzymology.

Application

• Biochemical Research: Used as a selective inhibitor of dihydrofolate reductase (DHFR) in enzymatic studies and metabolic pathway analysis.
• Pharmaceutical R&D: Serves as a key reference standard and intermediate in the development and testing of novel antifolate therapeutics.
• Mechanistic Studies: Employed to investigate the stereospecificity of drug action, transport, and resistance mechanisms in cell culture models.
• Academic Research: A vital tool in university and institutional labs for cancer research, immunology, and molecular biology projects.
• Analytical Standard: Used for method development, calibration, and quality control in HPLC, LC-MS, and other analytical techniques.

Basic Information

Product Name	D(-)-Amethopterin
CAS No.	51865-79-3
Molecular Formula	C₂₀H₂₂N₈O₅
Molecular Weight	454.44 g/mol
Synonyms	D-Methotrexate; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid; (-)-Methotrexate; D-(-)-Methotrexate; Amethopterin D-form; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, (S)-; NSC 407347; CCRIS 4011
EINECS	Contact for details

Quality Control

Our D(-)-Amethopterin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including chiral purity verification by HPLC, to ensure identity, potency, and consistency. A detailed Certificate of Analysis (COA) providing batch-specific results for assay, enantiomeric excess, and related substances is available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Enantiomeric Excess (Chiral HPLC)	≥ 99.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Related Substances (HPLC)	Individual impurity ≤ 0.5%; Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.