Description

Tris(2-Carboxyethyl)Phosphine Hydrochloride is a highly effective, water-soluble reducing agent widely used to cleave disulfide bonds in proteins and peptides. Its superior stability and selectivity compared to other reductants like dithiothreitol (DTT) make it a critical reagent for maintaining protein structure and function during analysis. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require reliable and consistent reduction of complex biomolecules.

Application

• Protein Biochemistry & Structural Analysis: Reduction and denaturation of proteins for SDS-PAGE, mass spectrometry, and chromatography.
• Therapeutic Antibody Development: Controlled reduction of interchain disulfide bonds in monoclonal antibodies (mAbs) for fragment generation and conjugation.
• Peptide Synthesis & Modification: Cleavage of protecting groups and reduction of disulfide bridges during solid-phase peptide synthesis (SPPS).
• Diagnostic Assay Manufacturing: Stabilization of protein conjugates used in ELISA, lateral flow assays, and other immunoassays.
• Cell Culture & Media Preparation: As a component of defined media to maintain redox potential and support cell growth.
• Nanoparticle Functionalization: Reduction of surface ligands for the controlled attachment of proteins or other biomolecules to gold or other nanoparticles.

Basic Information

Product Name	Tris(2-Carboxyethyl)Phosphine Hydrochloride
CAS No.	51805-45-9
Molecular Formula	C9H16ClO6P
Molecular Weight	286.65 g/mol
Synonyms	TCEP-HCl; Tris(2-carboxyethyl)phosphine hydrochloride; (2-Carboxyethyl)phosphine hydrochloride; TCEP hydrochloride; NSC 370681; Phosphine, tris(2-carboxyethyl)-, hydrochloride; Tris(carboxyethyl)phosphine hydrochloride; 2,2',2''-Phosphanetriyltriacetic acid hydrochloride
EINECS	Contact for details

Quality Control

Our Tris(2-Carboxyethyl)Phosphine Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research and production. Certificates of Analysis (COA) with detailed specifications are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
pH (1% Solution)	2.0 - 4.0
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.