Description

6-Aminofluorescein is a vital fluorescent dye derivative, widely recognized for its role as a key intermediate in the synthesis of advanced labeling reagents. Its primary value lies in its reactive amino group, which enables facile conjugation to biomolecules for creating highly sensitive detection probes. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and analytical chemistry sectors who require reliable, high-purity building blocks for fluorescent tagging and assay development.

Application

• Fluorescent Probe Synthesis: A critical precursor for creating amine-reactive fluorescent labels, such as FITC (Fluorescein Isothiocyanate) analogs and other fluorescein-based conjugates.
• Biomolecule Labeling: Used to tag antibodies, proteins, peptides, and nucleic acids for applications in flow cytometry (FACS), immunofluorescence (IF), and immunohistochemistry (IHC).
• Diagnostic Assay Development: Serves as a core component in the manufacture of reagents for ELISA, lateral flow assays, and other clinical diagnostic tests.
• Cell Biology & Microscopy: Enables the development of cell-permeable fluorescent dyes for live-cell imaging and intracellular tracking studies.
• Chemical Sensing: Acts as a building block for constructing chemosensors and indicators for detecting metal ions, pH changes, or specific enzymes.
• Material Science: Incorporated into polymers and nanoparticles to create functional fluorescent materials for security inks or advanced coatings.

Basic Information

Product Name	6-Aminofluorescein
CAS No.	51649-83-3
Molecular Formula	C20H13NO5
Molecular Weight	347.32 g/mol
Synonyms	6-Amino-3',6'-dihydroxyfluoran; 6-AF; 6-Aminofluoresceine; 2-(6-Amino-3-oxo-3H-xanthen-9-yl)benzoic acid; 6-Amino-3H-xanthen-3-one, 9-(2-carboxyphenyl)-; Fluorescein, 6-amino-; 6-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	Contact for details

Quality Control

Our 6-Aminofluorescein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and can support requirements for GMP or ISO-guided production upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/IR)	Conforms to standard
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.