Description

Naphtholas-Bi-B-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-D-galactosidase activity, a key enzyme in molecular biology and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies for applications in microbiology, cell biology, and high-throughput screening.

Application

• Enzymatic Substrate for β-D-Galactosidase: Used as a key reagent in assays to detect and measure β-D-galactosidase (lacZ) reporter gene expression in molecular biology and genetic engineering.
• Microbiological Testing: Employed in microbial identification and enumeration techniques, particularly for coliform bacteria in water and food safety testing.
• Diagnostic Kit Manufacturing: Serves as a critical component in the production of clinical and research diagnostic kits for enzyme-linked assays.
• Cell-Based Screening Assays: Utilized in high-throughput screening (HTS) platforms within pharmaceutical research to identify modulators of gene expression or enzyme activity.
• Histochemistry and Cytochemistry: Applied in laboratory staining procedures to localize β-galactosidase activity in tissue sections and cell cultures.
• Biochemical Research: Used in fundamental research to study enzyme kinetics, inhibition, and the mechanisms of glycoside hydrolases.

Basic Information

Product Name	Naphtholas-Bi-B-D-Galactopyranoside
CAS No.	51349-53-4
Molecular Formula	C22H24O11
Molecular Weight	464.42 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside; X-Gal; BCIG; 5-Bromo-4-chloro-3-indolyl β-D-galactoside; 4-Chloro-5-bromo-3-indolyl-β-D-galactopyranoside; Galactoside, 5-bromo-4-chloro-3-indolyl β-D-; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our Naphtholas-Bi-B-D-Galactopyranoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive analytical and diagnostic applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management standards, and our production facilities are designed to meet the rigorous demands of the life science industry.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccant and under inert atmosphere to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Enzymatic Activity	Yields intense blue chromogen upon cleavage by β-D-galactosidase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.