Description

Benzyloxycarbonylalanyl-Alanine Chloromethyl Ketone is a specialized synthetic peptide derivative designed for targeted biochemical research. This compound serves as a potent and irreversible active-site-directed inhibitor of serine and cysteine proteases, making it a critical tool for studying enzyme mechanisms and regulating proteolytic activity. It is primarily utilized by researchers in pharmaceutical development, biochemistry, and proteomics for inhibitor studies, enzyme characterization, and the development of therapeutic agents.

Application

• Protease Inhibition Studies: Acts as an active-site titrant and irreversible inhibitor for characterizing serine proteases (e.g., chymotrypsin, trypsin) and cysteine proteases.
• Biochemical Research: A key reagent for investigating enzyme kinetics, catalytic mechanisms, and substrate specificity in academic and industrial laboratories.
• Pharmaceutical Development: Used in the design and screening of potential therapeutic agents that target specific proteolytic pathways involved in diseases.
• Proteomics: Employed to selectively inhibit proteases in complex biological samples to stabilize protein profiles and prevent degradation during analysis.
• Enzyme Characterization: Essential for determining active enzyme concentration and for the functional analysis of novel proteases.

Basic Information

Product Name	Benzyloxycarbonylalanyl-Alanine Chloromethyl Ketone
CAS No.	51166-66-6
Molecular Formula	C21H23ClN2O5
Molecular Weight	418.87 g/mol
Synonyms	Z-Ala-Ala-CH2Cl; Z-Ala-Ala-CMK; Cbz-Ala-Ala-chloromethyl ketone; Benzyloxycarbonyl-L-alanyl-L-alanine chloromethyl ketone; N-Carbobenzoxyalanylalanine chloromethyl ketone; Ala-Ala-CK(Z); Protease Inhibitor Z-Ala-Ala-CMK; Nα-Cbz-L-alanyl-L-alanine chloromethyl ketone
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylalanyl-Alanine Chloromethyl Ketone is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and research reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.