Description

Coelenterazine H is a high-purity, synthetic luciferin derivative widely recognized as a critical substrate for Renilla luciferase. This compound matters for its exceptional role as a chemiluminescent probe, enabling highly sensitive detection and quantification in various bioanalytical assays. Researchers and quality control laboratories in the biotechnology, pharmaceutical, and life sciences sectors need it for applications ranging from gene expression reporting and cell viability assays to high-throughput screening and in vivo imaging.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: Serves as the light-emitting substrate to study protein-protein interactions and receptor-ligand binding in live cells.
• Reporter Gene Assays: Used with Renilla luciferase as a standard for dual-luciferase reporter systems, normalizing firefly luciferase signals for gene expression studies.
• In Vivo Bioluminescence Imaging (BLI): Enables real-time, non-invasive visualization of tumor growth, infection progression, and gene expression in small animal models.
• High-Throughput Screening (HTS): Provides a rapid, sensitive readout for drug discovery campaigns targeting various cellular pathways.
• ATP Detection and Cell Viability Assays: Utilized in kits that couple ATP concentration to luciferase activity, measuring metabolic health.
• Environmental Monitoring: Employed in biosensors for detecting specific pollutants or toxicants based on engineered luciferase reporter systems.

Basic Information

Product Name	Coelenterazine H
CAS No.	50909-86-9
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine Native; Coelenterazine, *Renilla*; *Renilla* Luciferin; 2-Deoxycoelenterazine; 6-(4-Hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-benzylimidazo[1,2-a]pyrazin-3(7H)-one; CLZN-h; CTZ-h
EINECS	Contact for details

Quality Control

Our Coelenterazine H is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. For long-term stability, desiccate. Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to pale yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescent Activity	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.