Description

Glutathione S-Transferase is a critical enzyme that catalyzes the conjugation of glutathione to a wide variety of electrophilic substrates, playing a central role in cellular detoxification and protection against oxidative stress. This enzymatic activity is fundamental for research in biochemistry, pharmacology, and toxicology, enabling the study of metabolic pathways and drug interactions. It is an essential reagent for pharmaceutical R&D, academic research institutions, and diagnostic kit manufacturers focused on biomarker discovery and metabolic studies.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study phase II metabolism, antioxidant defense mechanisms, and cellular response to xenobiotics.
• Pharmaceutical Drug Discovery: Employed in high-throughput screening (HTS) platforms to evaluate drug metabolism, identify potential drug-drug interactions, and assess compound toxicity profiles.
• Diagnostic Manufacturing: Serves as a critical component in the production of diagnostic kits for measuring GST activity as a biomarker for oxidative stress, liver function, and certain disease states.
• Toxicology Studies: Utilized in in vitro models to investigate the detoxification pathways of environmental toxins, carcinogens, and industrial chemicals.
• Enzyme Engineering & Biotechnology: Acts as a model enzyme for protein engineering studies aimed at enhancing stability, substrate specificity, or catalytic efficiency for industrial biocatalysis.

Basic Information

Product Name	Glutathione S-Transferase
CAS No.	50812-37-8
Molecular Formula	N/A (Protein)
Molecular Weight	~25-28 kDa (Isoform dependent)
Synonyms	GST; Glutathione Transferase; Glutathione Alkyltransferase; S-(Hydroxyalkyl)glutathione Lyase; RX:glutathione R-transferase; GSTase; EC 2.5.1.18
EINECS	Contact for details

Quality Control

Our Glutathione S-Transferase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analysis to ensure high specific activity, purity, and consistency. Certificates of Analysis (COA) are provided, detailing parameters such as protein concentration, specific activity (units/mg protein), and purity assessed by SDS-PAGE. We adhere to relevant quality standards suitable for research and in vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -70°C or below to prevent loss of activity. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	Lyophilized powder
Protein Content (Biuret)	> 90%
Specific Activity	> 30 units/mg protein
Purity (SDS-PAGE)	> 95%
Solubility	Soluble in water or buffer
Storage Buffer	Potassium phosphate, pH 6.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.