Description

H-Ala-Ala-Pna is a specialized peptide substrate designed for enzymatic research and biochemical applications. This compound serves as a critical tool for studying protease activity, particularly for enzymes that cleave at alanine residues. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require high-purity, well-characterized biochemical reagents. The product is supplied as a high-grade material suitable for sensitive assay development and kinetic studies.

Application

• Protease Enzyme Assays: Primary substrate for the kinetic characterization and inhibition studies of various proteases, including elastase and related serine proteases.
• Biochemical Research: Used in academic and industrial R&D laboratories to investigate enzyme mechanisms, substrate specificity, and catalytic efficiency.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and validate novel protease inhibitors as potential drug candidates.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits and reagents for clinical testing that measure protease levels.
• Quality Control in Bioprocessing: Used to monitor and ensure the activity and consistency of enzyme preparations used in industrial bioprocesses.

Basic Information

Product Name	H-Ala-Ala-Pna
CAS No.	50450-81-2
Molecular Formula	C16H22N4O5
Molecular Weight	350.37 g/mol
Synonyms	Ala-Ala-p-Nitroanilide; L-Alanyl-L-alanine 4-nitroanilide; N-(3-(2-Aminopropanamido)propanoyl)-4-nitroaniline; H-Ala-Ala-pNA; A9800; Substrate for Elastase; AAPNA; AAPF-pNA (related series)
EINECS	Contact for details

Quality Control

Our H-Ala-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) providing batch-specific data on identity, purity, and impurities is available for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.