Description

Tris(2-Carboxyethyl)Phosphine is a highly effective, water-soluble reducing agent widely used to cleave disulfide bonds in proteins and peptides. Its primary value lies in its superior stability and efficiency compared to other reductants like dithiothreitol (DTT), as it does not require a basic pH and is less prone to air oxidation. This makes it an indispensable reagent for researchers and manufacturers in the biotechnology and pharmaceutical industries, particularly for protein analysis, antibody-drug conjugate (ADC) development, and bioprocessing.

Application

• Protein Biochemistry & Research: Reduction of disulfide bonds in proteins and peptides for structural analysis, electrophoresis (SDS-PAGE), and mass spectrometry sample preparation.
• Therapeutic Antibody Development: Controlled reduction of interchain disulfides in monoclonal antibodies (mAbs) for fragment generation, conjugation, and antibody-drug conjugate (ADC) manufacturing.
• Bioprocessing & Cell Culture: Used in media formulations to maintain reducing environments and improve protein folding and yield in recombinant protein production.
• Diagnostic Assay Development: Stabilization and functionalization of proteins and enzymes used in immunoassays and biosensors.
• Polymer Chemistry: Acts as a chain transfer agent or reducing initiator in controlled radical polymerization processes.
• Material Science: Surface modification and functionalization of nanomaterials and gold nanoparticles for biomedical applications.

Basic Information

Product Name	Tris(2-Carboxyethyl)Phosphine
CAS No.	5961-85-3
Molecular Formula	C9H15O6P
Molecular Weight	250.19 g/mol
Synonyms	TCEP; TCEP Hydrochloride; Tris(2-carboxyethyl)phosphine hydrochloride; (2-Carboxyethyl)phosphine; [3-(Phosphanyl)propanoic acid]; Phosphine, tris(2-carboxyethyl)-; NSC 370363; TCEP•HCl
EINECS	227-736-5

Quality Control

Our Tris(2-Carboxyethyl)Phosphine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and biopharmaceutical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) due to its hygroscopic and easily oxidized nature. For long-term stability, desiccate if the container is opened frequently. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0% (Anhydrous basis)
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
pH (1% Solution)	2.0 - 4.0
Heavy Metals (as Pb)	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.