Description

n-Glutaryl-L-Phenylalanine p-Nitroanilide is a specialized chromogenic substrate designed for enzymatic assays. This compound matters for its high specificity and sensitivity in detecting and quantifying protease activity, particularly from enzymes like cathepsin C. It is primarily needed by research institutions, pharmaceutical developers, and diagnostic kit manufacturers engaged in biochemical research, drug discovery, and enzyme kinetics studies.

Application

• Protease Activity Assays: A key substrate for the spectrophotometric or fluorometric determination of dipeptidyl peptidase I (Cathepsin C) activity.
• Biochemical Research: Used in enzymatic studies to investigate inhibitor efficacy, enzyme kinetics, and substrate specificity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential drug candidates.
• Diagnostic Development: Serves as a critical component in the development of clinical diagnostic tests for conditions linked to abnormal protease levels.
• Enzyme Characterization: Facilitates the purification and functional analysis of proteases from various biological sources.

Basic Information

Product Name	n-Glutaryl-L-Phenylalanine p-Nitroanilide
CAS No.	5800-34-0
Molecular Formula	C20H21N3O6
Molecular Weight	399.40 g/mol
Synonyms	Glutaryl-Phe-pNA; Glutaryl-L-Phenylalanine 4-Nitroanilide; L-Phenylalanine, N-[4-[[(4-nitrophenyl)amino]carbonyl]-1-oxobutyl]-; GPNA; Cathepsin C Substrate; DPP-I Substrate; N-Glutaryl-L-phenylalanine-p-nitroanilide; N-(4-Nitrophenyl)-L-glutamyl-L-phenylalaninamide
EINECS	Contact for details

Quality Control

Our n-Glutaryl-L-Phenylalanine p-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to prevent degradation and moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 1.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.