Description

5-Bromo-6-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide is a specialized chromogenic substrate used in enzymatic detection and histochemical staining. This compound matters for its high sensitivity and specificity in revealing β-N-acetylglucosaminidase (NAGase) activity, a key biomarker in various biochemical and diagnostic assays. It is primarily needed by manufacturers and researchers in the life sciences, particularly for developing diagnostic kits, conducting cell biology research, and performing enzyme-linked immunosorbent assays (ELISAs).

Application

• Diagnostic Reagent Manufacturing: As a critical component in chromogenic substrates for clinical diagnostic kits detecting specific enzyme activities.
• Histochemistry & Cytochemistry: For the localization and visualization of β-N-acetylglucosaminidase activity in tissue sections and cell cultures.
• Biochemical Research: Used in enzyme kinetics studies and inhibitor screening assays within academic and pharmaceutical R&D laboratories.
• Microbiology: Potential application in microbial identification systems that utilize specific glycosidase profiles.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify modulators of glycosidase pathways relevant to disease.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide
CAS No.	5609-91-6
Molecular Formula	C16H18BrClN2O7
Molecular Weight	465.68 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl N-acetyl-β-D-glucosaminide; X-Nag (5-Br-6-Cl); 5-Br-6-Cl-3-Indolyl-β-D-GlcNAc; BCIP/INT Substrate Analog; NAGase Substrate; 3-Indolyl-β-D-GlcNAc derivative; Chromogenic NAG substrate; 5609-91-6
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl-n-Acetyl-β-D-Glucosaminide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and spectral analysis for structural confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider keeping the product desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.