Description

Phenyl-n-Acetyl-β-D-Glucosaminide is a specialized glycoside derivative, primarily used as a chromogenic substrate for enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise detection and quantification of specific enzyme activities. It is essential for biochemical research, diagnostic kit manufacturing, and pharmaceutical development, serving professionals in life sciences, clinical diagnostics, and industrial enzymology.

Application

• Enzyme Substrate for N-Acetyl-β-D-Glucosaminidase (NAGase): A key chromogenic substrate used in clinical and research assays to measure NAGase activity, a biomarker for various conditions.
• Diagnostic Reagent Manufacturing: Incorporated into kits for the detection of renal tubular damage, lysosomal storage disorders, and other pathologies.
• Biochemical Research: Utilized in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzymes.
• Pharmaceutical Quality Control: Applied in testing the activity and purity of enzyme-based pharmaceuticals and biologics.
• Microbiology and Cell Biology: Used as a tool to study glycosidase activity in bacteria, fungi, and mammalian cell cultures.
• Industrial Enzyme Analysis: Employed in process monitoring and quality assurance for industries utilizing glycosidase enzymes.

Basic Information

Product Name	Phenyl-n-Acetyl-β-D-Glucosaminide
CAS No.	5574-80-1
Molecular Formula	C14H19NO6
Molecular Weight	297.30 g/mol
Synonyms	4-Nitrophenyl N-acetyl-β-D-glucosaminide; p-Nitrophenyl N-acetyl-β-D-glucosaminide; PNP-NAG; NAG; 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; p-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside; N-Acetyl-β-D-glucosaminide 4-nitrophenyl; β-D-Glucopyranoside, 2-(acetylamino)-2-deoxy-, 4-nitrophenyl
EINECS	226-958-5

Quality Control

Our Phenyl-n-Acetyl-β-D-Glucosaminide is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by IR and NMR, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and consistent performance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	-30° to -35° (c=1 in H2O)
Solubility	Clear, colorless solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.