Description

3'-Chloromethotrexate is a chemically modified analog of the antifolate drug methotrexate, distinguished by the introduction of a chlorine substituent. This structural modification is of significant interest for its potential to alter biological activity, binding affinity, and metabolic stability compared to the parent compound. It serves as a critical pharmaceutical intermediate and research chemical for medicinal chemists and biologists investigating structure-activity relationships, targeted drug delivery, and novel therapeutic applications in oncology and immunology.

Application

• Pharmaceutical Intermediate: Key building block in the research and development of novel chemotherapeutic agents and targeted therapies.
• Biochemical Research: Used as a probe to study dihydrofolate reductase (DHFR) inhibition mechanisms and folate metabolism pathways.
• Medicinal Chemistry: Serves as a precursor for synthesizing more complex methotrexate derivatives with potentially enhanced efficacy or reduced toxicity.
• Academic & Institutional Research: Employed in university and laboratory settings for fundamental studies in cancer biology and drug resistance.
• Reference Standard: Utilized as an analytical standard in quality control laboratories for method development and validation.

Basic Information

Product Name	3'-Chloromethotrexate
CAS No.	5472-96-8
Molecular Formula	C₂₀H₂₂ClN₈O₅
Molecular Weight	489.89 g/mol
Synonyms	3'-Chloroamethopterin; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-3-chloro-L-glutamic Acid; 3'-Chloro-MTX; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]-3-chloropentanedioic acid; Chloromethotrexate (ambiguous); L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-3-chloro-
EINECS	Contact for details

Quality Control

Our 3'-Chloromethotrexate is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets exacting research-grade specifications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting batch-specific results. We support cGMP-compliant manufacturing for advanced applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.