Description

3-Maleimido-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is a specialized spin-labeling reagent featuring a maleimide functional group and a stable nitroxide radical. This compound is critical for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein structure, dynamics, and conformational changes. It is primarily utilized by researchers and manufacturers in the fields of structural biology, biophysics, and pharmaceutical development for probing biomolecular interactions.

Application

• Site-Directed Spin Labeling (SDSL) for Electron Paramagnetic Resonance (EPR) spectroscopy studies.
• Probing protein structure, folding, and conformational dynamics in solution.
• Investigation of membrane protein topology and lipid interactions.
• Studying nucleic acid structure and protein-nucleic acid complexes.
• Development of molecular sensors and probes for diagnostic applications.
• Use as a cross-linking agent in the synthesis of advanced polymeric materials.
• Research into radical polymerization processes and polymer characterization.

Basic Information

Product Name	3-Maleimido-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy
CAS No.	5389-27-5
Molecular Formula	C13H19N2O3
Molecular Weight	251.30 g/mol
Synonyms	3-Maleimido-PROXYL; MTSSL; (1-Oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)maleimide; 3-Maleimido-2,2,5,5-tetramethyl-1-pyrrolidinyloxy free radical; 3-Maleimido-PROXYL spin label; 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxamide; 3-Maleimidomethyl-PROXYL; Maleimido-TEMPO
EINECS	Contact for details

Quality Control

Our 3-Maleimido-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot is analyzed to confirm identity, purity, and radical content. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. Keep the container in a dry, cool, and well-ventilated area. Allow the product to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
EPR Signal	Characteristic triplet spectrum
Melting Point	Contact for details
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.